1.Question or problem to be


The aim of this research
proposal is to perform phytochemical screening of a new variety of Syzygium
cumini. Syzygium caryophylli folium (family-
Myrtaceae) is an evergreen tropical tree and native to the Indian Subcontinent
and was reported from Chandrapur district of Vidarbha region, Maharashtra. The
main objective is to determine phytochemicals present in S.caryophylli folium
leaves through ethanol extract along with biological activity against gram
positive and gram negative microorganisms.

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2.Background to the problem


Medicinal plants are used for
healing and curing of human diseases due to presence of phytochemical
constituents. Phytochemicals are of
two categories; primary and secondary constituents. Primary constituents
include chlorophyll, proteins sugar and amino acids while secondary
constituents contain terpenoids and alkaloids phenolic compounds. Terpenoids
exhibit various pharmacological activities such as anti-malarial, anti-fungal,
anti-inflammatory activities etc. Alkaloids are used as anaesthetic agents.
Phenolic compounds possess biological properties such as anti-ageing,
anti-carcinogen, anti-inflammation, cardiovascular protection and improvement
(Han et al.,2007).


products have been a part of phytomedicines since time immemorial. These can be
derived from any part of the plant like barks, stems, leaves, roots, seeds, etc
(Cragg and David, 2001), i.e., any part of the plant may contain active
compounds which occur naturally. The plants belonging to the family Myrtaceae
are used as anti-inflammatory, antinociceptive, antimicrobial, antioxidant
agents. This species are also used as folk medicines to treat several diseases,
especially gastrointestinal disorders, haemorrhagic and infectious diseases.



to this fact, preliminary phytochemical
screening of plants is in need to discover and develop novel therapeutic drugs
with improved efficacy. Numerous research groups have also reported such
studies throughout the world. Correlation
between the phytoconstituents and the bioactivity of plant is desirable to know
for the synthesis of compounds with specific activities to treat various health
ailments and chronic diseases.



to address the problem


fresh leaves of the plant Syzygium
caryophylli folium should be washed
thoroughly with water, shade dried for about 10 days. The dried plant sample
then should be homogenized into fine powder and stored in air sealed containers
at room temperature before extraction. The components of finely powdered plant
material are to be extracted using Soxhlet apparatus using organic solvent
ethanol. The extract then is concentrated and stored in air tight glass
container for further use. The aqueous extract of leaves sample is analysed for
the presence of the phytochemical constituents present in the plant.


The ethanolic extract is to be evaporated to dryness in boiling water bath.
Then about 0.2 grams of residue is dissolved in 2% H2SO4  and filtered. A few drops of Dragondroff’s
reagent is added. Orange-red precipitates indicates the presence of alkaloids.

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