Development of high public presentation fixed movie bioreactor for intervention of methylbenzene vaporAbstraction:The present survey is an effort to develop a new type of bioreactor to get the better of the restriction like clogging, limited mass transportation of hydrophobic VOCs, O restriction, growing rate of bugs normally associated with conventional biofilters. A freshly designed bioreactor referred as fixed movie bioreactor ( FFB ) has been developed for intervention of toluene vapor. The cogent evidence of construct utilizing a research lab paradigm revealed that the FFB greatly surpasses the public presentation of conventional gas-phase bioreactors.

Experimental consequences showed a toluene riddance capacity every bit high as 358.6 gmethylbenzenem-3reactorH-1with a removal efficiency of 95 % at a gas abode clip of 1.3 min and a toluene recess concentration of 6.

3-7.8 gram-3. Overall, consequences show that the public presentation of FFB far exceeds that of presently used bioreactors for air pollution control.Keywords: Fixed Film Bioreactor ; Toluene ; Biofilters

  1. Introduction

Biological waste air intervention engineering makes usage of several types of bioreactors depending on the burden and sort of pollutant to be treated. The type of bioreactor used for suspension has a direct effect on efficiency of the intervention procedure.

Bioreactors play a really of import function in the control of VOCs and odorous gases that are emitted by fouling industries. Although a figure of different coni¬?gurations exist, the chief types of conventional air stage biological reactors include bioi¬?lters, biotrickling i¬?lters and bioscrubbers [ 7 ] .Extensive research exists on biofilters, biotrickling filters and the usage of revolving biological contactors [ 3,8 ] and possible betterments of these techniques in the signifier of membrane biofiter, foamed emulsion based bioreactor [ 4,5,6,9 ] etc. Unfortunately, these new developments have merely resulted in modest betterments of the public presentation over bing techniques. Hence, recent research attempts has been towards development of fresh bioreactors, which can get the better of the disadvantages associated with conventional systems and are able to efficaciously degrade the volatile compounds at higher concentration and lading rates with shorter keeping clip.In this paper, we describe the characteristics of a freshly developed novel reactor referred to as the fixed movie bioreactor ( FFB ) . This paper discusses increased efficiency of FFB for the intervention of compounds characterized by high H2O solubility and high volatility, in footings of higher O mass transportation into the liquid medium incorporating the pollutant, higher interfacial country per unit reactor volume and higher microbic biofilm stableness.

  1. Materials and Methods
    1. Fixed Film Bioreactor ( FFB )bench-scale unit

A conventional representation of the FFB bench-scale unit developed for intervention of toluene vapor is shown in Figure 1. FFB was fabricated by chromium steel steel with a on the job volume of 40L. FFB consists of nylon rope as a substrate for the microbic movie. The ropes ( 412mm long, 8mm midst ) placed horizontally supported on two round phonograph record ( 164mm diameter ) with an interior rope spacing of 15mm.

The full assembly was mounted on a stiff chromium steel steel shaft and placed in a rectangular armored combat vehicle. The surface country provide by nylon ropes was about 1.17 m2which corresponded to specific surface country 167 m2/m3FFB volume. The submerging of the nylon ropes was approximately 50 % and shaft was revolving at fixed 3 revolutions per minute by changeless velocity motor. The FFB armored combat vehicle was fitted with two noses for the recess and mercantile establishment provender solution severally.A metered watercourse of toluene vapor was introduced through the gas sparger while the on the job volume of FFB dwelling of mineral medium and the active civilization.

Toluene degrading pool used in the experiments was obtained from a biofilter used for intervention of methylbenzene bluess. The composing of mineral medium is ( in gL?1in distilled H2O ) K2HPO4– 0.615, KH2Polonium4– 0.

385, MgSO46H2O – 0.25, NH4NO3– 1, NaCl – 1 and CaCl2– 0.026 ; the pH of the medium was adjusted to 7.0±0.1 utilizing dilute HCl ( 0.1 N ) .

  1. Analytic Methods

A side gas watercourse ( 0.

5 Lmin?1for 10 min ) from the recess and mercantile establishment of FFB was sporadically absorbed in 20 milliliter methyl alcohol, and 5?l of methyl alcohol was injected into a gas chromatograph ( GC ) for analysis. It was found that this method adequately averaged short-run fluctuations in the recess and mercantile establishment concentrations and resulted in greater truth over direct injection of grab gaseous samples. Coincident analysis of selected gas samples collected at recess and mercantile establishment port confirms no false positive consequences for debasement and therefore minimizes the mistakes. The GC ( Perkin Elmer Clarus-5000, USA ) was equipped with a i¬‚ame ionisation sensor ( FID ) and a DB-5 capillary column ( 0.025 mm?30 m, fused silicon oxide ) . The gathered recess and mercantile establishment liquid samples of FFB were i¬?rst i¬?ltered utilizing 0.2?m i¬?lter and their soluble entire organic C ( TOC ) concentrations were measured by a TOC analyzer ( Thermo Electron Corporation’s, Model: TOC 1200 ) .

[ 1 ] . A bench-top pH metre was used for supervising the pH of liquid samples. The CO2concentration in the mercantile establishment gas watercourse of the bioi¬?lter was measured utilizing the infrared CO2detector MI 70 ( Vaisala, Finland ) . Plating was used to recite entire and specii¬?c VOC degrading bacterial population as per the criterion methods [ 1 ] . For specii¬?c VOC degrading counts, home bases were incubated in a methylbenzene atmosphere.

  1. Consequences and Discussion


1 Overall FFB public presentation3.1.1. Startup and Continuous Operation of FFBFigure 2 shows the public presentation of FFB towards methylbenzene remotion during uninterrupted operation for 104 yearss, while Table 1 describes the conditions during each experimental stage. At the beginning of experiment, a figure of steps were taken in order to perchance better methylbenzene riddance. Ceaseless mineral medium supply to FFB shows removal efficiency started to increase after 3 yearss of uninterrupted operation stage. Performance reached a comparatively steady value at about 95 % remotion after 6 yearss.FFB in general, more hard to get down than biofilters because the procedure civilization needs to attach to a bearer, organize a biofilm to get the better of wash-out, and is capable to shear emphasis.

Removal of methylbenzene by physical soaking up into the liquid was expected to be the major mechanism of remotion for the i¬?rst 4 yearss based on gas-liquid equilibrium computations. Metabolites of methylbenzene were non detected from twenty-four hours 3 onward indicating that some biological activity was already happening in the FFB. Still, it took longer than expected to make a steady remotion of methylbenzene, and the RE did non make 100 % as expected.

From twenty-four hours 21 onward ( Phase 2 ) , the FFB was operated at toluene recess concentrations of 0.6-1 gram-3, and a sustained RE of about 90 % was observed. This corresponds to a toluene riddance capacity ( EC ) of 43-44 gmethylbenzenemreactor-3H-1. Subsequently, the aim was to find the methylbenzene EC at high burdens to measure the maximal capacity of the FFB, and therefore, the methylbenzene recess concentration was later increased during Phase 3 and Phase 4.

The methylbenzene RE reached a steady value of about 95 % , with a maximal methylbenzene riddance capacity of 279.3 gmethylbenzenemreactor-3H-1. During Phases 5-6, the reactor was subjected to higher methylbenzene burdens through addition recess concentration at lower EBRT. This was done to detect the distribution of intermediates, which during the earlier stages showed that most of the methylbenzene removed, was converted to CO2, the intended terminal merchandise as observed in TOC profiling of liquid medium of FFB. Throughout the survey, the DO and TOC of the liquid remained in the scope of 6-8 milligram L-1and 10-20 milligram L-1severally, proposing somewhat oxidising conditions and efficient remotion of methylbenzene. TOC analyses of the liquid revealed that 85-90 % of the organic C Federal to FFB as methylbenzene was used, proposing that the EBRT is optimal for efficient toluene debasement. FFB subjected to high organic burdens has non experienced clogging jobs even at surplus suspended biomass growing which is normally observed with biotrickling i¬?lters [ 2 ] .The uninterrupted operation of the reactor for 104 yearss without any signii¬?cant loss of activity is an of import i¬?nding.

3.1.2. Steady-State Performance and rating of FFBSteady province public presentation of FFB was evaluated by comparing with steady province public presentation of biofilter operated on same province of status to acquire a realistic fake theoretical account of industrial scenario. Figure 3.A shows the methylbenzene EC vs. burden for the FFB at steady province operation.

The FFB was able to take & amp ; gt ; 95 % methylbenzene at lading up to 300 g m-3H-1at EBRT of 1.3 min and no discovery was observed even at higher lading up to 370 g m-3H-1. The additive addition in elimination capacity of reactor with addition in lading consequence shows that FFB has non yet reached its maximal EC even lading at 358.6 gram-3H-1.These observations provide infinite for the betterment of public presentation of FFB at higher burden and low EBRT. The EC of FFB found maximal and stable as comparison with EC reported for froth emulsion bioreactor system for intervention of methylbenzene [ 4 ] .Steady province public presentation of biofilter operated on same status shows nonlinear correlativity between lading and riddance capacity after lading more than 150 gram-3H-1( Figure.3.

B ) . Significant loss in toluene removal efficiency was observed for biofillter operated at higher lading show insufficiency of biofilter to handle toluene concentration above 3 gram-3H-1.3.1.

3 Culture FeaturesIn order to develop an account to some of the ascertained phenomena, a basic word picture of the procedure civilization was attempted utilizing simple plating techniques. Plate numeration allows one to quickly measure the microbiota of FFB. The consequences in Figure 4 shows, the suspended civilization in liquid media of FFB consist of both the specific methylbenzene debasers and other heterotrophs which are able to absorb the metabolic intermediates of toluene debasement. The methylbenzene degrader’s concentration additions with addition in toluene burden.

The ratio of methylbenzene debasers to entire heterotrophs in suspended civilization increased to 0.5 toward the Phase 6 shows substrate dependent growing of microbiota. The ratio allows assuming the presence of about 50-55 % specific methylbenzene debasers immobilized on the rope along with entire heterotrophs. The entire C content of FFB media found in scope of 10-20 ppm for toluene burden of about 350 g m3H-1.

TOC consequences indicate towards important mineralization of methylbenzene in FFB.

  1. Decision

A novel improved immobilized bioreactor referred to as a Fixed Film Bioreactor was developed for remotion of methylbenzene bluess. Experiments utilizing bench scale paradigm unit shows effectual mineralization of methylbenzene and has overcome the restrictions associated with bing bioreactors. Under selected conditions, public presentations every bit high as 95 % remotion at toluene riddance capacities of 358.6 g m-3H-1were achieved.

Under these conditions, both O and pollutant-substrate restrictions non occurred. Such high toluene-elimination public presentation is far superior to any antecedently study for conventional or fresh bioreactors for air pollution control. The full potency of the FFB has non yet been explored. For illustration, the FFB could turn out to be a really effectual apparatus for handling contaminated air with assorted toxic compounds.

However, the following challenge will be measuring FFB public presentation for handling mixture of VOC’s at high burden.RecognitionsThe writers would wish to appreciatively admit the fiscal support provided by Mitsui Environmental Engineering Trust ( MEET ) , New Delhi for transporting out this work. The support from MSc undertaking trainee pupils during this work is punctually acknowledged.Mention

  1. American Public Health Association, American Water Works Association and Water Environment Federation ( APHA, AWWA, WEF ) , Standard Methods for the Examination of Water and Wastewater, 19th erectile dysfunction. Washington, DC, USA ( 2005 )
  2. Cox HH J, Deshusses MA. 1998. Biological waste air intervention in biotrickling filters. Curr Opin Biotechnol 9:256–262.

  3. Devinny JS, Deshusses MA, Webster TS. 1999. Biofiltration for air pollution control. New York: CRC Lewis Publishers. 10–15.
  4. Kan E, Deshusses MA.

    2005. Development of Foamed Emulsion Bioreactor for Air Pollution Control. Biotechnology and Bioengineering 84 ( 2 ) : 240-244.

  5. Kumar A, Dewulf J, Langenhove HV. 2008a. Membrane-based biological waste gas intervention. Chemical Engineering Journal 136: 82–91.

  6. Kumar A, Luvsanjamba JDM, Langenhove HV. 2008b. Continuous operation of membrane bioreactor handling toluene bluess by Burkholderia vietnamiensis G4. Chemical Engineering Journal 14:193–200.
  7. Mudliar S, Giri B, Padoley K, Satpute D, Dixit R, Bhatt P, Pandey R, Juwarkar A, Vaidya A. 2010.

    Bioreactors for intervention of VOCs and odours – A reappraisal. Journal of Environmental Management 91:1039–1054

  8. Patwardhan AW. 2003. Revolving biological contactors: a reappraisal.

    Ind Eng Chem Res 42: 2035–2051.

  9. Shareefdeen Z, Singh A. 2005. Biotechnology for Odor and Air Pollution Control Springer, Berlin, Heidelberg, New York.

Table 1Operating status for FFB during the different experimental stage

Phase Time( yearss ) Toluene Inlet Concentration( g m-3­) Toluene Loading( g m-3H-1) Elimination capacity( g m-3H-1) EBRT( min ) Removal efficiency( % ) Liquid provender make up /day
1 0-25 0.0-0.55 18-21 21 1.8 75-95 % 20 %
2 26-38 0.

6- 1.0

25-46 43-44 1.3-1.5 85-95 % 20 %
3 39-57 1-1.8 47-85 78 1-1.3 90-95 % 20 %
4 58-66 1.


90-166 164.8 1.3 & A ; gt ; 95 20 %
5 67-78 5.


200-300 279.3 1.3 & A ; gt ; 95 20 %
6 79-104 6.3-7.8 300-370 358.6 1.3 & A ; gt ; 95 20 %
pH of FFB liquid during survey = 6.

5-7.4Dissolved oxygen concentration = 6.49-7.65 milligram cubic decimeter-1Temperature of FFB liquid = 24.


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