The Purpose of making this experiment is “how factors affect on the rate of enzyme activity. ” “We examine the affects of enzyme activity when exposing enzymes to different substrates. PH and temperature. In this experiment a type of enzyme called Peroxides has been used. Peroxidase is the enzymes are found in life being so. it can be founded in both liver and murphy. For mensurating the rate of enzyme activity in each experiment the tallness of the bubbling froth column formed by the reaction’s merchandise have been measured by millimeter. within a 60 seconds. Then the units changed to mm/h. Rate is distance over clip and it means how fast something occurs in a given clip period. The enzyme peroxidase in two signifiers of murphy peroxidase and liver peroxidase has been settled in two different state of affairss. ( different PHs: 3. 7. 11 ) . ( different temperature: cold. warm and hot ) and in other experiment mixes with three different substrates: ( H2O. H2O2. glucose solution ) . The information collected during the experiment brand treatment about hypothesizes. *
The verification of the substrate for peroxides hypothesis: Hydrogen peroxide would be the best substrate for peroxidase and the reaction between liver peroxidase and H peroxide will be stronger than murphy peroxidase and H peroxide. Peroxidase facilitates the “breakdown of their substrate metabolically produce toxic H peroxide. ” The reaction is: 2H2O2 & A ; enzyme ? 2H20+O2 & A ; enzyme. H2O2 works as substrate. H2O2 is strong oxidant. That it is considered a extremely reactive O spices. Besides. the available peroxidase in murphy and liver has the same map as with oxidase. Sing to the map of liver. which is filtrating. there should be tonss of O groups in liver. A Potato is a storage tissue comparing to the liver has less free O groups so the reaction of murphy peroxidase with H2O2 is slower than the liver peroxidase.
In other point of position. among proposal substrates in this experiment such as H2O. glucose solution and H2O2. H peroxide increases the concentration of O2 and H2O ( consequence of transforming of H peroxide ) when it places following to peroxidase liver instead than when it places following to peroxidase murphy. Therefore. when concentration additions. the strength of reaction addition. The information besides showed the same consequences. Adding 3ml of H2O2 to the murphy peroxide gave 41 millimeter of froth tallness and the enzyme rate was 2460 mm/h. Adding 3ml of H2O2 to liver peroxidase gave 151 millimeter froth tallness and 9060 mm/h as enzyme rate. When H2O and glucose solution added to peroxidase in liver and murphy. any reaction happened so. no bubbles created. The hypothesis has been approved in first experiment. *
The consequence of temperature on peroxidase activity hypothesis: As the temperature increases. the enzyme activities will increase until it reaches the optimal temperature scope. and after that the enzyme activities will diminish. Meanwhile. liver peroxidase activity due to increasing temperature should be more than potato peroxidase activity. Increasing temperature at the beginning. do the enzyme activity additions because there is more energy to rush up the reaction. Then. the enzyme activities start to diminish because the alterations to active site due to the temperature will no longer fit substrate. The information collected during the experiment showed more activity in murphy peroxidase at 5 C as cold temperature instead than 35 C as warm temperature and it did non hold any reaction when the enzyme placed in hot temperature 68 C. with increasing temperature the murphy peroxidase lost its activity.
The first portion of the hypothesis failed because based on the hypothesis it supposed to be seen more activity in murphy enzyme at 35 C instead than 5 C. but it did non go on. It can be guessed that Potato lives under the land where there is non adequate sunshine and as a consequence it is colder than surface of the land and murphy enzymes are active in low temperature and it will lose its activity when the temperature be higher. But. the experiment about the liver peroxidase approved the hypothesis. Liver had wow reaction at 6 C as cold temp and at 33 C as warm temp excessively. And it had no activity at 64 C at wholly. As it is an enzyme found in the organic structure. the optimal temperature would normally be body temperature around 37 C so. high activity of liver peroxidase at 33 C is expectable. But. high activity at 6 C is unusual.
Possibly an mistake occurred during the experiment such as non reading the temperature right or the room temperature ( which is optimum ) effects on the reaction during the experiment or possibly the clip reader made error in puting clip. However. the 64 C is perfectly non optimum because the construction of enzyme and its active site has been destroyed in high temperature. Sing to the 2nd portion of the hypothesis. 9060 mm/H rate of enzyme activity in liver peroxidase at 6 and 33 grade of Celsius showed more activity in comparison to 2100 mm/H and 1980 mm/H of murphy peroxidase at 5 and 35 grade of Celsius. So 2nd portion of hypothesis has been approved.
The consequence of PH on peroxidase activity hypothesis:
Increasing pH causes increasing the activity of the enzyme until it reaches optimal pH scope and after this it will diminish. About potato peroxidase as the pH additions. the activity of the enzyme will increase until it reaches optimal pH scope. which could be around pH 7. because the enzyme is less denaturized when it reaches the preferable pH degree. and after this it will diminish because the active site will alter in form and it will no longer accept substrates. The experiment showed 2400 mm/H rate of activity for murphy peroxidase in PH 7 and 2220 mm/H rate of activity in PH 3 and 2040 mm/H rate of activity in PH 11. The consequence perfectly confirmed the hypothesis. PH7 & gt ; PH3 & gt ; PH11 About liver peroxidase. increasing PH cause increasing enzyme activity until it reaches optimal pH scope but as liver usage to populate in a buffer state of affairs the differences in activities due to alterations in PH can non be much a batch. It does non intend that it is able to work in any PH state of affairs.
Liver besides has an optimum scope and does non work out of that scope. In this experiment the outlook was high activity around PH 7. which is near to the PH of organic structure. 7. 5. But 882 mm/H rate of activity in PH11 was the most active state of affairs. Besides 842 mm/H in PH7 was more than 828 mm/H in PH3. Then liver peroxidase activity increased by increasing PH degree and it means that Hypothesis has been failed. PH11 & gt ; PH7 & gt ; PH3 Sing to the informations collected by the category some of the hypotheses have been approved and the others have been failed. Data tabular array for verification of substrate for peroxidase showed that in both instances. murphy and liver. H2O2 was the best substrate among H2O and glucose and H peroxide. Besides the enzyme activity of liver ( 9019 mm/H ) was more than enzyme activity of murphy ( 2245 mm/H ) . The difference was so obvious in the graph.
So. the hypothesis about substrate approved. The informations collected by the category about how temperature consequence on enzyme activity of murphy and the graph showed that enzyme activity decreased by increasing the temperature. Therefore. the hypothesis is non true about murphy enzyme. But. the information of the category supported the hypothesis about the liver peroxidase. The graph besides showed how increasing temperature effects on liver enzymes. The liver enzymes got more activity in warm temperature comparing to the cold temperature and it was perfectly inactive in hot temperature. The informations collected by the category about how PH consequence on murphy peroxidase showed that in PH7 enzyme activity of the murphy is still the highest degree of activity but the information showed more activity in PH11 than PH3. Although this portion is different of what did our group acquire in this experiment but the hypothesis is still true. As graph showed. 8645 mm/H in PH11 and 7525 mm/H in PH7 and 6640 mm/H in PH3. the enzyme activity in liver increased when degree of PH increased and it was besides against of the hypothesis in this experiment merely like what did our group go. Many mistakes may hold been occurred in this experiment.
The state of affairs and status were non the same for all groups. For illustration there were no specific definition for the words “ cold. warm or hot” . Any group may mensurate the tallness of the bubbles in any temperature as cold or warm or hot. There may even be mistakes in infixing the information in the table online. Because some of the information is wholly out of scope and out of suggestion. However. PH. temperature and even the substrate consequence on enzymes activity. An enzyme has particular active site and particular substrate can be attached to it. The optimum scope is of import in enzyme activity. The enzyme is less active when it gets under or above the degree of optimum scope. And it is true about both PH and temperature.
In our existent life we come across with many state of affairss that can consequence on the enzymes whether interior or out of our organic structure. For illustration in the human digestive piece of land. there are enzymes runing at basic pH ( oral cavity and bowel ) and extremely acidic pH ( tummy ) . If the sourness of the tummy extremely increases the enzymes are non able to work any more. Or. some people have some job to digest dairy because of enzyme lack. Or. if a kid has fever the enzymes of his/her organic structure won’t be able to work any more and it causes purging.